Bolton was shot multiple times after he pulled a car over at Cottonwood and Perkins around 9:18 p.m. The suspect is still at large, according to police.
A citizen witness used Bolton's radio to call for help. Bolton was rushed to the Regional Medical Center in critical condition. He was later pronounced dead.
Memphis Police Director Toney Armstrong and Mayor A C Wharton held a press conference outside the Regional Medical Center Saturday night.
"There's a theme that black lives matter, and at the end of the day, we have to ask ourselves, do all lives matter, regardless of race, creed, color, economic status, what profession a person holds? All lives matter. And this is just a reminder of just of dangerous this job is," Armstrong said.
Bolton's death is the third police killing in the line of duty in four years. In July 2011, Officer Timothy Warren was shot and killed at the DoubleTree Hotel downtown. And in December 2012, Officer Martoiya Lang was fatally shot while serving a warrant at a home in East Memphis.
Memphis Police officer Sean Bolton was shot and killed during a traffic stop Saturday night in Parkway Village.